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Transcription profiling of yeast wild type and ggc1-delta strains after treatment with rapmycin

ABSTRACT: This experiment was a 4-part timeseries (or, 2x2x4 factorial design) designed to provide additional biological insight into the role of ggc1 in the rapamycin response of Saccharomyces cerevisiae. Treated and untreated cultures were compared at 0, 1, 2, and 4 hours of growth, in both WT and ggc1-delta backgrounds.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Ariel Paulson

PROVIDER: E-TABM-638 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Delayed correlation of mRNA and protein expression in rapamycin-treated cells and a role for Ggc1 in cellular sensitivity to rapamycin.

Fournier Marjorie L ML Paulson Ariel A Pavelka Norman N Mosley Amber L AL Gaudenz Karin K Bradford William D WD Glynn Earl E Li Hua H Sardiu Mihaela E ME Fleharty Brian B Seidel Christopher C Florens Laurence L Washburn Michael P MP

Molecular & cellular proteomics : MCP 20091110 2

To identify new molecular targets of rapamycin, an anticancer and immunosuppressive drug, we analyzed temporal changes in yeast over 6 h in response to rapamycin at the transcriptome and proteome levels and integrated the expression patterns with functional profiling. We show that the integration of transcriptomics, proteomics, and functional data sets provides novel insights into the molecular mechanisms of rapamycin action. We first observed a temporal delay in the correlation of mRNA and prot ...[more]

PMID: 19955083

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