Unknown,Transcriptomics,Genomics,Proteomics

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Ribonucleoprotein immunoprecipitation of fission yeast to study myosin proteins and other cytoskeletal components

ABSTRACT: Ribonucleoprotein immunoprecipitation microarray (RIp-chip) study using various myosin proteins and other cytoskeletal components from the fission Schizosaccaromyces pombe

ORGANISM(S): Schizosaccharomyces pombe

SUBMITTER: Juan Mata 

PROVIDER: E-TABM-656 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Genome-wide mapping of myosin protein-RNA networks suggests the existence of specialized protein production sites.

Mata Juan J  

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 20091005 2

Motor proteins can organize posttranscriptional processes by transporting ribonucleoprotein complexes to specific locations. To investigate a possible role of myosin proteins in gene expression control, I have identified mRNAs associated with five myosin heavy chains in the fission yeast Schizosaccharomyces pombe, by purifying the proteins and identifying bound transcripts using DNA microarrays. Each myosin coimmunoprecipitated with 5-13 different mRNAs (approximately 0.1-0.2% of all genes), inc  ...[more]

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