Proteomics

Dataset Information

0

The endosomal sorting adaptor HDPTP is required for ephrinB EphB signalling in cell collapse and motor axon guidance. Sci Rep, 2019


ABSTRACT: Spectral count files from EphB2 BioID experiment found in "The endosomal sorting adaptor HD-PTP is required for ephrin-B:EphB signalling in motor axon guidance.". The parameters and protocol are described in detail in the methods section. In brief, we over expressed BirA* C-terminal tagged EphB2 in HEK 293 cells. We stimulated these cells with either media (no ligand), Fc or ephrin-B2-Fc in order to capture the protein landscape of ephrin-B2:EphB2 forward signalling. BirA*-Flag-EGFP controls are VL_20150825_COT_05_AK.raw, VL_20150825_COT_06_AK.raw, VL_20150825_COT_07_AK.raw, VL_20150825_COT_08_AK.raw Empty Vector controls are VL_20151116_COT_01_HEK293_pcDNA5EV_AK.raw, VL_20151116_COT_02_HEK293_pcDNA5EV_AK.raw, VL_20151116_COT_03_HEK293_pcDNA5EV_AK.raw, VL_20151116_COT_04_HEK293_pcDNA5EV_AK.raw EphB2_Fc-BirA*-Flag samples are VL_20160623_COT_01_AK.raw, VL_20160718_COT_01_AK.raw, VL_20161116_COT_05_AK.raw, VL_20161116_COT_06_2_AK.raw EphB2_NoLigand-BirA*-Flag samples are VL_20160630_COT_01_AK.raw, VL_20160630_COT_02_AK.raw, VL_20161116_COT_03_AK.raw, VL_20161116_COT_04_AK.raw EphB2_EB2-BirA*-Flag samples are VL_20160623_COT_02_AK.raw, VL_20160718_COT_02_AK.raw, VL_20161118_COT_01_AK.raw, VL_20161118_COT_02_AK.raw

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Artur Kania  

PROVIDER: MSV000083410 | MassIVE | Wed Feb 06 10:55:00 GMT 2019

REPOSITORIES: MassIVE

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2014-04-01 | GSE28334 | GEO
2014-04-01 | E-GEOD-28334 | biostudies-arrayexpress
| S-EPMC6379420 | biostudies-literature
2018-11-09 | MSV000083125 | MassIVE
| S-EPMC5855093 | biostudies-literature
| S-EPMC6697728 | biostudies-literature
| S-EPMC3628632 | biostudies-literature
2024-01-30 | GSE223738 | GEO
2019-10-20 | MSV000084477 | MassIVE
2019-03-21 | MSV000083608 | MassIVE