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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of individual yeast wild type strains vs a pool of wild type strains to estimate biological variation

ABSTRACT: Contains a collection of wildtype Saccharomyces cerevisiae strains for estimating the biological variation. Wildtypes are obtained from the yeast wildtypes - wt pool background set HybSet, by randomly taking 100 wt vs. refpool (pooled wts) and 100 refpool vs. wt hybridizations

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Patrick Kemmeren

PROVIDER: E-TABM-773 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Project description:Contains a collection of wildtype Saccharomyces cerevisiae strains for estimating the biological variation. Wildtypes are obtained from the yeast wildtypes - wt pool background set HybSet, by randomly taking 100 wt vs. refpool (pooled wts) and 100 refpool vs. wt hybridizations Two channel microarrays were used. RNA isolated from a large amount of wt yeast from a single culture was used as a common reference. Two independent cultures were hybridized on two separate microarrays.

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Project description:Contains a collection of wildtype matA Saccharomyces cerevisiae strains for estimating the biological variation. Please refer to a related experiment where the yeast strains were grown in a platereader: E-MTAB-1351 (http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-1351). Two other wildtype pool experiments have also been deposited in ArrayExpress previously. The experiments have similar setup as the current submission and E-MTAB-1351 (e.g. using matAlpha strains and also grown either using an erlenmeyer or using the Tecan platereader), but the pools are much larger and also used more frequently. The ArrayExpress accessions are: E-TABM-773 (matAlpha wildtype strains erlenmeyer growth, http://www.ebi.ac.uk/arrayexpress/experiments/E-TABM-773) and E-TABM-984 (matAlpha wildtype strains, tecan platereader growth, http://www.ebi.ac.uk/arrayexpress/experiments/E-TABM-984)

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