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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Methylation and microRNA profiling of human paediatric glioma cell lines

ABSTRACT: Constitutive signalling pathway activation is a key feature of primary tumours and cancer cell lines. The regulation of gene expression changes may be via both genomic and epigenetic means, and understanding the mechanisms by which signal transduction may be activated can provide rational targets for therapeutic intervention. We have previously carried out DNA copy and expression profiling on a unique panel of five paediatric glioma cell lines, and have noted only a limited influence of gene amplification on gene expression. In the present study we have extended our work to include a measure of global methylation changes as well as micro RNA profiling in the same cell lines. We noted various instances of signalling pathway activation associated with specific hypermethylation or miRNA regulation of gene expression at various effectors also observed in primary paediatric tumours. These data provide evidence for the multifaceted nature of gene expression changes in paediatric high grade glioma, and identify novel targets for targeted therapy in this treatment refractory disease.

ORGANISM(S): Homo sapiens

SUBMITTER: Anita Grigoriadis

PROVIDER: E-TABM-858 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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