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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of mouse embryonic stem cells exposed to cisplatin at four time points and concentrations to investigate DNA damage signalling pathways

ABSTRACT: To gain insight in the kinetics and interplay of the predominant transcriptional responses of DNA damage signalling pathways in undifferentiated cells, mouse embryonic stem cells were exposed to cisplatin at four different time points (2, 4, 8 and 24 hr) and concentrations (1, 2, 5 and 10 uM). RNA was isolated and subjected to genome-wide expression profiling.

ORGANISM(S): Mus musculus

SUBMITTER: Jan Polman

PROVIDER: E-TABM-89 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A portrait of cisplatin-induced transcriptional changes in mouse embryonic stem cells reveals a dominant p53-like response.

Kruse Jacqueline J C M JJ Svensson J Peter JP Huigsloot Merei M Giphart-Gassler Micheline M Schoonen Willem G E J WG Polman Jan E M JE Jean Horbach G G van de Water Bob B Vrieling Harry H

Mutation research 20070202 1-2

Accumulation of damage in undifferentiated cells may threaten homeostasis and regenerative capacity. Remarkably, p53 has been suggested to be transcriptionally inactive in these cells. To gain insight in the kinetics and interplay of the predominant transcriptional responses of DNA damage signalling pathways in undifferentiated cells, mouse embryonic stem cells were exposed to cisplatin at four different time points (2, 4, 8 and 24h) and concentrations (1, 2, 5 and 10 microM). RNA was isolated a ...[more]

PMID: 17327130

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