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Chromatin immunoprecipitation of mouse pancreatic beta-cells.

ABSTRACT: To gain insights into how pancreatic beta-cells are programmed in vivo, we profiled key histone methylations (H3K4/K27me3) in embryonic stem cells, multipotent progenitors of the nascent embryonic pancreas, purified beta-cells, and 10 other adult tissues (all under normal, untreated conditions). For these cells we also purified RNA to analyze tissue specfic genome wide transcription levels in relation to histone modifications. Corresponding RNA microarrays can be found under accession E-TABM-877.

ORGANISM(S): Mus musculus

SUBMITTER: Joris van Arensbergen

PROVIDER: E-TABM-906 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Derepression of Polycomb targets during pancreatic organogenesis allows insulin-producing beta-cells to adopt a neural gene activity program.

van Arensbergen Joris J García-Hurtado Javier J Moran Ignasi I Maestro Miguel Angel MA Xu Xiaobo X Van de Casteele Mark M Skoudy Anouchka L AL Palassini Matteo M Heimberg Harry H Ferrer Jorge J

Genome research 20100415 6

The epigenome changes that underlie cellular differentiation in developing organisms are poorly understood. To gain insights into how pancreatic beta-cells are programmed, we profiled key histone methylations and transcripts in embryonic stem cells, multipotent progenitors of the nascent embryonic pancreas, purified beta-cells, and 10 differentiated tissues. We report that despite their endodermal origin, beta-cells show a transcriptional and active chromatin signature that is most similar to ec ...[more]

PMID: 20395405

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