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Unknown,Transcriptomics,Genomics,Proteomics

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Chromatin immunoprecipitation of transcription factor binding in yeast cells perturbed with methyl-methanesulfonate

ABSTRACT: Genome-wide promoter-binding analysis of transcription factors in S.cerevisiae cells perturbed with 0.03% methyl-methanesulfonate for 60 minutes

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Chris Workman

PROVIDER: E-TABM-92 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A systems approach to mapping DNA damage response pathways.

Workman Christopher T CT Mak H Craig HC McCuine Scott S Tagne Jean-Bosco JB Agarwal Maya M Ozier Owen O Begley Thomas J TJ Samson Leona D LD Ideker Trey T

Science (New York, N.Y.) 20060501 5776

Failure of cells to respond to DNA damage is a primary event associated with mutagenesis and environmental toxicity. To map the transcriptional network controlling the damage response, we measured genomewide binding locations for 30 damage-related transcription factors (TFs) after exposure of yeast to methyl-methanesulfonate (MMS). The resulting 5272 TF-target interactions revealed extensive changes in the pattern of promoter binding and identified damage-specific binding motifs. As systematic f ...[more]

PMID: 16709784

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