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Transcription profiling by array of mouse Paneth cells from Atg16l1 mutant and wild type strains

ABSTRACT: Transcription profiling of Paneth cells from Atg16l1hm mice with CR6 virus. Normalized data is provided as an additional file on the FTP site for this experiment.

ORGANISM(S): Mus musculus

SUBMITTER: Chad Storer

PROVIDER: E-TABM-958 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcription profiling of Paneth cells from Atg16l1hm mice with CR6 virus

Project description:Transcription profiling of Paneth cells from Atg16l1hm mice with CR6 virus

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Microarray of laser capture microdissected Paneth cells from Atg16l1 T300A and wildtype littermates mice that were either exposed or not with mainstream cigarette smoking for 6 weeks

Project description:We found that mainstream cigarette smoking (4 cigarettes/day, 5 days/week for 2 weeks using Kentucky Research Cigarettes 3R4F) resulted in >20% decrease in the percentage of normal Paneth cell population in Atg16l1 T300A mice but showed minimal effect wildtype littermate control mice, indicating that Atg16l1 T300A polymorphism confers sensitivity to cigarette smoking-induced Paneth cell damage. We performed transcriptional profiling to identify molecular mechanisms associated with Paneth cell defect in Atg16l1 T300A mice exposed to cigarette smoking. Female mice were used at 4-5 weeks of age. Cigarette smoking was performed using smoking chamber with the dosage and schedule as described above. The Paneth cells of the mice were harvested by laser capture microdissection for transcriptomic analysis after completing 6 weeks of smoking.

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