Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling by array of mouse treated with Antiotensin II to induce hypertension in either a chronic or acute fashion

ABSTRACT: Spotted arrays and Affymetrix Genechip mouse430_2 were hybridized with the same LabeledExtracts in order to compare results from the two methodologies. The LabeledExtracts were derived from mice treated with Antiotensin II to induce hypertension in either a chronic or acute fashion.

This experiment was reloaded into ArrayExpress in December 2010 after six separate biosources were created from the original two biosources tigr.org:musage:BioSource_AFX-HTA-SA-1 and tigr.org:musage:BioSource_AFX-HTA-SA-2.

ORGANISM(S): Mus musculus

SUBMITTER: Joe White

PROVIDER: E-TIGR-121 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Independence and reproducibility across microarray platforms.

Larkin Jennie E JE Frank Bryan C BC Gavras Haralambos H Sultana Razvan R Quackenbush John J

Nature methods 20050421 5

Microarrays have been widely used for the analysis of gene expression, but the issue of reproducibility across platforms has yet to be fully resolved. To address this apparent problem, we compared gene expression between two microarray platforms: the short oligonucleotide Affymetrix Mouse Genome 430 2.0 GeneChip and a spotted cDNA array using a mouse model of angiotensin II-induced hypertension. RNA extracted from treated mice was analyzed using Affymetrix and cDNA platforms and then by quantita ...[more]

PMID: 15846360

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Project description:The definition of biomarkers correlating with disease progression could have impact on the rational design of novel diagnostic approaches for bovine tuberculosis in cattle. We have used the advantages of the murine model to identify promising candidates in the host transcriptome post-infection. RNA from BALB/c splenocytes and lung cells after aerogenic Mycobacterium bovis infection were with high-density microarrays to defining biomarkers of disease progression. In antigen-stimulated splenocytes we found statically significant modulation of 1109 genes early after infection and 1134 at later time-point post-infection. 618 of these genes were modulated at both time points. In the lung 282 genes were significantly modulated post-infection. Amongst the most strongly up-regulated genes were granzyme A in spleen, and cxcl9, granzyme B, interleukin-22, interluekin-17A receptor, and ccr6 in lungs. The expression of 20 of the most up-regulated genes identified in the murine studies were evaluated using PBMC from uninfected and naturally infected cattle. We could show that the expression of cxcl9, granzyme A and interleukin-22 following in vitro stimulation with PPD was significantly increased in infected cows compared to naïve animals. Thus, we have demonstrated that murine transcriptome analysis can be used to predict responses in cattle. In addition, we have prioritised CXCL9, GnzA and IL-22 as potential additional readout systems for the ante-mortem diagnosis of bovine tuberculosis. Two groups of 5 BALB/c mice each were infecetd with approx 600 CFU M. bovis via the intranasal route. At days 3 and 14 post challenge five mice per group were euthanized and spleens and lungs harvested. One group of 5 mice were used as a control. Their splenocytes and lung cells were stimulated in vitro for 3 days with M7 protein pool. Antigen cell culture stimulations in mice were performed using an equal pool of seven secreted, immunogenic recombinant mycobacterial proteins (Rv1886c, Rv3019c, Rv3763, Rv3804c, Rv0251, Rv0287 and Rv0288) common to M. bovis and BCG, referred here as M7 protein cocktail. Each protein was used at final concentration of 2μg/ml in 3-day culture.

Dataset Information

Transcription profiling by array of mouse treated with Antiotensin II to induce hypertension in either a chronic or acute fashion

Publications

Independence and reproducibility across microarray platforms.

Similar Datasets

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