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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of human HUVEC (Human Umbilical Vein Endothelial Cells) cells exposed to heat shock (42 degrees Celcius) for different time points (1hr, 3 hrs, 6 hr and 12 hrs) and vs non heat-shock cells with varying amounts of external spikes added

ABSTRACT: HUVEC (Human Umbilical Vein Endothelial Cells) cells were exposed to heat shock (42 degrees Celcius) for different time points (1hr, 3 hrs, 6 hr and 12 hrs) and compared to non heat-shock cells. External normalization controls were added in equal amounts to equivalent amounts of total RNA.

ORGANISM(S): Homo sapiens

SUBMITTER: Dik van Leenen

PROVIDER: E-UMCU-2 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Monitoring global messenger RNA changes in externally controlled microarray experiments.

van de Peppel Jeroen J Kemmeren Patrick P van Bakel Harm H Radonjic Marijana M van Leenen Dik D Holstege Frank C P FC

EMBO reports 20030401 4

Expression profiling is a universal tool, with a range of applications that benefit from the accurate determination of differential gene expression. To allow normalization using endogenous transcript levels, current microarray analyses assume that relatively few transcripts vary, or that any changes that occur are balanced. When normalization using endogenous genes is carried out, changes in expression levels are calculated relative to the behaviour of most of the transcripts. This does not refl ...[more]

PMID: 12671682

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