Ontology highlight
ABSTRACT:
ORGANISM(S): Homo sapiens
SUBMITTER: Branko Braam
PROVIDER: E-UMCU-7 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Hypertension (Dallas, Tex. : 1979) 20050207 4
Shear stress modulates gene expression in endothelial cells (ECs) partly through nitric oxide (NO), acting via enhanced cGMP formation by guanylyl cyclase (GC). We addressed non-cGMP-mediated transcriptional responses to shear stress in human umbilical ECs subjected to high-laminar shear stress (25 dyn/cm2; 150 minutes). RNA was isolated, reverse-transcribed, Cy3/5-labeled, and hybridized to 19 K human microarrays. High shear (n=6), high shear with 100 micromol/L L-NAME (n=3), and high shear wit ...[more]