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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of human HUVEC cells exposed to 250 uM DETA-NONOate for 4 hours in the presence and absence of 10 uM of the guanylate cyclase inhibitor 1H-[1,2,4]oxadiazolo-[4,3,2]quinoxalin-1-one (ODQ)

ABSTRACT: HUVECs were exposed to 250 uM DETA-NONOate for 4 hours in the presence and absence of 10 uM of the guanylate cyclase inhibitor 1H-[1,2,4]oxadiazolo-[4,3,2]quinoxalin-1-one (ODQ). Control cells were exposed to vehicle (methanol 0.1%).

ORGANISM(S): Homo sapiens

SUBMITTER: Branko Braam

PROVIDER: E-UMCU-7 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Nitric oxide-dependent and nitric oxide-independent transcriptional responses to high shear stress in endothelial cells.

Braam Branko B de Roos Remmert R Bluyssen Hans H Kemmeren Patrick P Holstege Frank F Joles Jaap A JA Koomans Hein H

Hypertension (Dallas, Tex. : 1979) 20050207 4

Shear stress modulates gene expression in endothelial cells (ECs) partly through nitric oxide (NO), acting via enhanced cGMP formation by guanylyl cyclase (GC). We addressed non-cGMP-mediated transcriptional responses to shear stress in human umbilical ECs subjected to high-laminar shear stress (25 dyn/cm2; 150 minutes). RNA was isolated, reverse-transcribed, Cy3/5-labeled, and hybridized to 19 K human microarrays. High shear (n=6), high shear with 100 micromol/L L-NAME (n=3), and high shear wit ...[more]

PMID: 15699468

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