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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of human HUVEC cells exposed for 2, 4, 8 and 24 hours to (Z)-1-[N-(2-aminoethyl)-N-(2-ammonioethyl)amino]diazen-1-ium-1,2-diolate (DETA-NONOate) 250 uM

ABSTRACT: A time-course experiment was performed: HUVECs were exposed for 2, 4, 8 and 24 hours to (Z)-1-[N-(2-aminoethyl)-N-(2-ammonioethyl)amino]diazen-1-ium-1,2-diolate (DETA-NONOate) 250 uM.

ORGANISM(S): Homo sapiens

SUBMITTER: Branko Braam

PROVIDER: E-UMCU-8 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Nitric oxide-dependent and nitric oxide-independent transcriptional responses to high shear stress in endothelial cells.

Braam Branko B de Roos Remmert R Bluyssen Hans H Kemmeren Patrick P Holstege Frank F Joles Jaap A JA Koomans Hein H

Hypertension (Dallas, Tex. : 1979) 20050207 4

Shear stress modulates gene expression in endothelial cells (ECs) partly through nitric oxide (NO), acting via enhanced cGMP formation by guanylyl cyclase (GC). We addressed non-cGMP-mediated transcriptional responses to shear stress in human umbilical ECs subjected to high-laminar shear stress (25 dyn/cm2; 150 minutes). RNA was isolated, reverse-transcribed, Cy3/5-labeled, and hybridized to 19 K human microarrays. High shear (n=6), high shear with 100 micromol/L L-NAME (n=3), and high shear wit ...[more]

PMID: 15699468

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