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Biocompatible Short-Peptides Fibrin Co-assembled Hydrogels.

ABSTRACT: Fibrin hydrogels made by self-assembly of fibrinogen obtained from human plasma have shown excellent biocompatible and biodegradable properties and are widely used in regenerative medicine. The fibrinogen self-assembly process can be triggered under physiological conditions by the action of thrombin, allowing the injection of pregel mixtures that have been used as cell carriers, wound-healing systems, and bio-adhesives. However, access to fibrinogen from human plasma is expensive and fibrin gels have limited mechanical properties, which make them unsuitable for certain applications. One solution to these problems is to obtain composite gels made of fibrin and other polymeric compounds that improve their mechanical properties and usage. Herein, we prepared composite hydrogels made by the self-assembly of fibrinogen together with Fmoc-FF (Fmoc-diphenylalanine) and Fmoc-RGD (Fmoc-arginine-glycine-aspartic acid). We have shown that the mixture of these three peptides co-assembles and gives rise to a unique type of supramolecular fiber, whose morphology and mechanical properties can be modulated. We have carried out a complete characterization of these materials from chemical, physical, and biological points of view. Composite gels have improved mechanical properties compared to pure fibrin gels, as well as showing excellent biocompatibility ex vivo. In vivo experiments have shown that these gels do not cause any type of inflammatory response or tissue damage and are completely resorbed in short time, which would enable their use as vehicles for cell, drug, or growth factor release.

SUBMITTER: Gila-Vilchez C

PROVIDER: S-EPMC10013376 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature

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Biocompatible Short-Peptides Fibrin Co-assembled Hydrogels.

Gila-Vilchez Cristina C Mañas-Torres Mari Carmen MC García-García Óscar Darío ÓD Escribano-Huesca Alfredo A Rodríguez-Arco Laura L Carriel Víctor V Rodriguez Ismael I Alaminos Miguel M Lopez-Lopez Modesto Torcuato MT Álvarez de Cienfuegos Luis L

ACS applied polymer materials 20230221 3

Fibrin hydrogels made by self-assembly of fibrinogen obtained from human plasma have shown excellent biocompatible and biodegradable properties and are widely used in regenerative medicine. The fibrinogen self-assembly process can be triggered under physiological conditions by the action of thrombin, allowing the injection of pregel mixtures that have been used as cell carriers, wound-healing systems, and bio-adhesives. However, access to fibrinogen from human plasma is expensive and fibrin gels ...[more]

PMID: 36935654

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Project description:The persistent challenge of biofouling, driven by the accumulation of microorganisms and biological residues on surfaces, undermines operational efficiency and safety across multiple industries. Functionalized peptide based biocompatible and supramolecular coating can provide a substantial solution to this crucial issue. This present study describes the formation of polydopamine-comprised sticky tubes through the co-assembly of an antifouling peptide P1 (FF-PFB) and Polydopamine Nanoparticles (PDA NPs) with an adhesive catechol moiety. To overcome the synthetic complications associated with the attachment of adhesive l-DOPA or dopamine with antifouling peptides, we have employed a simple co-assembly strategy. These co-assembled sticky tubes form a stable, biocompatible coating on desired surfaces (glass and aluminium) and resist fouling. The design consists of a diphenylalanine-based antifouling peptide covalently coupled with pentafluoro benzaldehyde (PFB), which could self-assemble into a stable functional coating through the adhesive catechol moiety of PDA NPs. This functional coating effectively resists bacterial and protein adhesion. These sticky tubes coated desired surfaces (glass and aluminium) exhibit excellent antifouling activity against both tested Gram (+)ve (S. aureus) and Gram (-)ve (E. coli) bacterial strains. More importantly, this simple co-assembly and drop-coating method has significant promise, primarily attributed to its simplicity of operation, which reduces production costs and expands the potential for widespread commercialization. This study not only contributes to the fundamental understanding of the antifouling process but also offers a practical and sustainable solution to the challenges caused by biofouling. Our findings, achieved through the simple and effective co-assembly strategy with two different functional components, pave the way for developing promising antifouling materials with broad applications in industries where effective biofouling resistance is crucial.

Dataset Information

Biocompatible Short-Peptides Fibrin Co-assembled Hydrogels.

Publications

Biocompatible Short-Peptides Fibrin Co-assembled Hydrogels.

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