Dataset Information

In vivo measurement of the Young's modulus of the cell wall of single root hairs.

ABSTRACT: Root hairs are cells from the root epidermis that grow as long tubular bulges perpendicular to the root. They can grow in a variety of mechanical or chemical environments. Their mechanical properties are mainly due to their stiff cell wall which also constitutes a physical barrier between the cell and its environment. Thus, it is essential to be able to quantify the cell wall mechanical properties and their adaptation to environmental cues. Here, we present a technique we developed to measure the Young's (elastic) modulus of the root hair cell wall. In essence, using custom-made glass microplates as cantilevers of calibrated stiffness, we are able to measure the force necessary to bend a single living root hair. From these experiments one can determine the stiffness and Young's modulus of the root hair cell wall.

SUBMITTER: Pereira D

PROVIDER: S-EPMC10015226 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature

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In vivo measurement of the Young's modulus of the cell wall of single root hairs.

Pereira David D Alline Thomas T Schoenaers Sébastjen S Asnacios Atef A

Cell surface (Amsterdam, Netherlands) 20230301

Root hairs are cells from the root epidermis that grow as long tubular bulges perpendicular to the root. They can grow in a variety of mechanical or chemical environments. Their mechanical properties are mainly due to their stiff cell wall which also constitutes a physical barrier between the cell and its environment. Thus, it is essential to be able to quantify the cell wall mechanical properties and their adaptation to environmental cues. Here, we present a technique we developed to measure th ...[more]

PMID: 36938503

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Project description:Static Young's modulus (E s) is a critical property required in numerous petroleum calculations. Various models to forecast E s have been proposed in the literature. However, existing models, by and large, lack precision and are confined to specific data set ranges. This study proposes an alternative approach for E s determination, utilizing different machine learning methods, such as an adaptive neuro-fuzzy inference system (ANFIS). In these proposed methods, the predictor variables include bulk formation density (RHOB), shear wave velocity (DTs), and compressional wave velocity (DTc). The models were trained on a data set comprising 1853 hydrocarbon reservoir rock samples from globally diverse locations. They were evaluated using trend, group error, and statistical error analyses. To test the efficacy of the proposed models, the optimally performing model was identified and used to detect the rock types along with the previously published models. Results indicated that ANFIS is the optimum model and can predict E s with an average absolute percentage relative error (AAPRE) of 5.1% and a correlation coefficient (R) of 0.9602. The ANFIS method has some benefits over other machine learning approaches insofar as its superiority in reaching a quicker decision about the mapped relationship between the inputs and outputs because it combines artificial neural networks and fuzzy logic in one tool. The ANFIS can perform a highly nonlinear mapping and displays a better learning ability. The proposed ANFIS model demonstrates its ability to capture accurate physical relationships between input rock properties and E s through trend analysis, which shows that increasing the RHOB increases the E s. Contrarily, increasing the DTc and DTs reduces the E s. Furthermore, the ANFIS model can accurately detect the rock types based on its E s determinations. This research demonstrates the importance of accurately predicting E s for the proper identification of rock types. Thus, this study offers potential advancements in geological assessments of hydrocarbon reservoirs and improvements in many petroleum engineering applications.

Dataset Information

In vivo measurement of the Young's modulus of the cell wall of single root hairs.

Publications

In vivo measurement of the Young's modulus of the cell wall of single root hairs.

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