Engineered CRISPR prime editors with compact, untethered reverse transcriptases.
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ABSTRACT:
SUBMITTER: Grunewald J
PROVIDER: S-EPMC10023297 | biostudies-literature | 2023 Mar
REPOSITORIES: biostudies-literature
Publications
Engineered CRISPR prime editors with compact, untethered reverse transcriptases.
Grünewald Julian J Miller Bret R BR Szalay Regan N RN Cabeceiras Peter K PK Woodilla Christopher J CJ Holtz Eliza Jane B EJB Petri Karl K Joung J Keith JK
Nature biotechnology 20220926 3
The CRISPR prime editor PE2 consists of a Streptococcus pyogenes Cas9 nickase (nSpCas9) fused at its C-terminus to a Moloney murine leukemia virus reverse transcriptase (MMLV-RT). Here we show that separated nSpCas9 and MMLV-RT proteins function as efficiently as intact PE2 in human cells. We use this Split-PE system to rapidly identify and engineer more compact prime editor architectures that also broaden the types of RTs used for prime editing. ...[more]
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