Dataset Information

Functional and Proteomic Insights into Aculeata Venoms.

ABSTRACT: Aculeate hymenopterans use their venom for a variety of different purposes. The venom of solitary aculeates paralyze and preserve prey without killing it, whereas social aculeates utilize their venom in defence of their colony. These distinct applications of venom suggest that its components and their functions are also likely to differ. This study investigates a range of solitary and social species across Aculeata. We combined electrophoretic, mass spectrometric, and transcriptomic techniques to characterize the compositions of venoms from an incredibly diverse taxon. In addition, in vitro assays shed light on their biological activities. Although there were many common components identified in the venoms of species with different social behavior, there were also significant variations in the presence and activity of enzymes such as phospholipase A₂s and serine proteases and the cytotoxicity of the venoms. Social aculeate venom showed higher presence of peptides that cause damage and pain in victims. The venom-gland transcriptome from the European honeybee (Apis mellifera) contained highly conserved toxins which match those identified by previous investigations. In contrast, venoms from less-studied taxa returned limited results from our proteomic databases, suggesting that they contain unique toxins.

SUBMITTER: Dashevsky D

PROVIDER: S-EPMC10053895 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Functional and Proteomic Insights into Aculeata Venoms.

Dashevsky Daniel D Baumann Kate K Undheim Eivind A B EAB Nouwens Amanda A Ikonomopoulou Maria P MP Schmidt Justin O JO Ge Lilin L Kwok Hang Fai HF Rodriguez Juanita J Fry Bryan G BG

Toxins 20230316 3

Aculeate hymenopterans use their venom for a variety of different purposes. The venom of solitary aculeates paralyze and preserve prey without killing it, whereas social aculeates utilize their venom in defence of their colony. These distinct applications of venom suggest that its components and their functions are also likely to differ. This study investigates a range of solitary and social species across Aculeata. We combined electrophoretic, mass spectrometric, and transcriptomic techniques t ...[more]

PMID: 36977115

Similar Datasets

Project description:The response of communities to environmental change is expected to vary among feeding guilds. To evaluate the response of guilds to environmental factors without considering the taxonomic specificities, it is useful to examine Aculeata bees and wasps, which consist of closely related taxa including different guilds, pollinators, predators, and parasitoids. In this study, we evaluated changes in species diversity (SD) and functional traits of each feeding guild along an elevational gradient in a boreal forest in northern Japan. We used yellow pan traps to collect Aculeata bees and wasps at 200-1600 m above sea level. We investigated six functional traits (trophic level, seasonal duration, body size, elevational range, nesting position, and soil dependency) and the horizontal distribution of the species. The SD of all Aculeata, predators, and parasitoids decreased with an increase in elevation; however, the SD of pollinators did not show any specific trend. Although the functional trait composition of all Aculeata species did not show any trend, that of each feeding guild responded to elevation in different ways. Pollinators increased in body size and showed a decrease in seasonal duration with increasing elevation, suggesting that tolerance and seasonal escape from physical stress at high elevations are important for shaping pollinator communities. Predators increased their elevational range and the proportion of above-ground nesting species increased with increasing elevation, suggesting that the ability to live in a wider range of environments and avoid unsuitable soil environments at high elevations might be important. Parasitoids changed their hosts and displayed variable traits with increasing elevation, suggesting that brood parasitoids have difficulty in surviving at high elevation. The traits for each guild responded in different ways, even if they were dominated by the same environmental factors. Our findings imply that differences in the responses of functional traits would produce different community assembly patterns in different guilds during further climate change.

Project description:BackgroundMacauba (Acrocomia aculeata) is a non-domesticated neotropical palm that has been attracting attention for economic use due to its great potential for oil production comparable to the commercially used oil palm (Elaeis guineensis). The discovery of associations between quantitative trait loci and economically important traits represents an advance toward understanding its genetic architecture and can contribute to accelerating macauba domestication. Pursuing this advance, this study performs single-trait and multi-trait GWAS models to identify candidate genes associated with vegetative and oil production traits in macauba. Eighteen phenotypic traits were evaluated from 201 palms within a native population. Genotyping was performed with SNP markers, following the protocol of genotyping-by-sequencing. Given that macauba lacks a reference genome, SNP calling was performed using three different strategies: using i) de novo sequencing, ii) the Elaeis guineenses Jacq. reference genome and iii) the macauba transcriptome sequences. After quality control, we identified a total of 27,410 SNPs in 153 individuals for the de novo genotypic dataset, 10,444 SNPs in 158 individuals using the oil palm genotypic dataset, and 4,329 SNPs in 167 individuals using the transcriptome genotypic dataset. The GWAS analysis was then performed on these three genotypic datasets.ResultsStatistical phenotypic analyses revealed significant differences across all studied traits, with heritability values ranging from 63 to 95%. This indicates that the population contains promising genotypes for selection and the initiation of breeding programs. Genetic correlations between the 18 traits ranged from -0.47 to 0.99. The total number of significant SNPs in the single-trait and multi-trait GWAS was 92 and 6 using the de novo genotypic dataset, 19 and 11 using the oil palm genotypic dataset, and 1 and 2 using the transcriptome genotypic dataset, respectively. Gene annotation identified 12 candidate genes in the single-trait GWAS and four in the multi-trait GWAS, across the 18 phenotypic traits studied, in the three genotypic datasets. Gene mapping of the macauba candidate genes revealed similarities with Elaeis guineensis and Phoenix dactylifera. The candidate genes detected are responsible for metal ion binding and transport, protein transportation, DNA repair, and other cell regulation biological processes.ConclusionsWe provide new insights into genomic regions that map candidate genes associated with vegetative and oil production traits in macauba. These potential candidate genes require confirmation through targeted functional analyses in the future, and multi-trait associations need to be scrutinized to investigate the presence of pleiotropic or linked genes. Markers linked to traits of interest could serve as valuable resources for the development of marker-assisted selection in macauba for its domestication and pre-breeding.

Dataset Information

Functional and Proteomic Insights into Aculeata Venoms.

Publications

Functional and Proteomic Insights into Aculeata Venoms.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets