Ontology highlight
ABSTRACT:
SUBMITTER: Warsinger-Pepe N
PROVIDER: S-EPMC10085756 | biostudies-literature | 2023 Apr
REPOSITORIES: biostudies-literature
Warsinger-Pepe Natalie N Chang Carly C Desroberts Connor R CR Akbari Omar S OS
G3 (Bethesda, Md.) 20230401 4
Heat-shock-inducible expression of genes through the use of heat-inducible promoters is commonly used in research despite leaky expression of downstream genes of interest without targeted induction (i.e. heat shock). The development of non-leaky inducible expression systems is of broad interest for both basic and applied studies, to precisely control gene expression. Here we characterize the use of Polycomb response elements and the inducible Heat-shock protein 70Bb promoter, previously describe ...[more]