Unknown

Dataset Information

0

Structural basis of spike RBM-specific human antibodies counteracting broad SARS-CoV-2 variants.


ABSTRACT: The decrease of antibody efficacy to mutated SARS-CoV-2 spike RBD explains the breakthrough infections and reinfections by Omicron variants. Here, we analyzed broadly neutralizing antibodies isolated from long-term hospitalized convalescent patients of early SARS-CoV-2 strains. One of the antibodies named NCV2SG48 is highly potent to broad SARS-CoV-2 variants including Omicron BA.1, BA.2, and BA.4/5. To reveal the mode of action, we determined the sequence and crystal structure of the Fab fragment of NCV2SG48 in a complex with spike RBD from the original, Delta, and Omicron BA.1. NCV2SG48 is from a minor VH but the multiple somatic hypermutations contribute to a markedly extended binding interface and hydrogen bonds to interact with conserved residues at the core receptor-binding motif of RBD, which efficiently neutralizes a broad spectrum of variants. Thus, eliciting the RBD-specific B cells to the longitudinal germinal center reaction confers potent immunity to broad SARS-CoV-2 variants emerging one after another.

SUBMITTER: Shitaoka K 

PROVIDER: S-EPMC10088672 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature

altmetric image

Publications


The decrease of antibody efficacy to mutated SARS-CoV-2 spike RBD explains the breakthrough infections and reinfections by Omicron variants. Here, we analyzed broadly neutralizing antibodies isolated from long-term hospitalized convalescent patients of early SARS-CoV-2 strains. One of the antibodies named NCV2SG48 is highly potent to broad SARS-CoV-2 variants including Omicron BA.1, BA.2, and BA.4/5. To reveal the mode of action, we determined the sequence and crystal structure of the Fab fragme  ...[more]

Similar Datasets

| EMPIAR-10999 | biostudies-other
| S-EPMC11228396 | biostudies-literature
| S-EPMC9774083 | biostudies-literature
| S-EPMC10071473 | biostudies-literature
| S-EPMC7723407 | biostudies-literature
| S-EPMC7558967 | biostudies-literature
| S-EPMC8908544 | biostudies-literature
| S-BSST649 | biostudies-other
| S-EPMC9229035 | biostudies-literature
| S-EPMC9400479 | biostudies-literature