Ontology highlight
ABSTRACT:
SUBMITTER: Develtere W
PROVIDER: S-EPMC10123101 | biostudies-literature | 2023 Apr
REPOSITORIES: biostudies-literature
Develtere Ward W Waegneer Evelien E Debray Kevin K De Saeger Jonas J Van Glabeke Sabine S Maere Steven S Ruttink Tom T Jacobs Thomas B TB
Nucleic acids research 20230401 7
Multiplex amplicon sequencing is a versatile method to identify genetic variation in natural or mutagenized populations through eco-tilling or multiplex CRISPR screens. Such genotyping screens require reliable and specific primer designs, combined with simultaneous gRNA design for CRISPR screens. Unfortunately, current tools are unable to combine multiplex gRNA and primer design in a high-throughput and easy-to-use manner with high design flexibility. Here, we report the development of a bioinfo ...[more]