Direct-seq: programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen.
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ABSTRACT:
SUBMITTER: Song Q
PROVIDER: S-EPMC7278172 | biostudies-literature | 2020 Jun
REPOSITORIES: biostudies-literature
Publications
Direct-seq: programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen.
Song Qingkai Q Ni Ke K Liu Min M Li Yini Y Wang Lixia L Wang Yingying Y Liu Yingzheng Y Yu Zhenxing Z Qi Yinyao Y Lu Zhike Z Ma Lijia L
Genome biology 20200608 1
CRISPR-based genome perturbation provides a new avenue to conveniently change DNA sequences, transcription, and epigenetic modifications in genetic screens. However, it remains challenging to assay the complex molecular readouts after perturbation at high resolution and at scale. By introducing an A/G mixed capture sequence into the gRNA scaffold, we demonstrate that gRNA transcripts could be directly reverse transcribed by poly (dT) primer together with the endogenous mRNA, followed by high-con ...[more]
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