Suppressing Kaposi's Sarcoma-Associated Herpesvirus Lytic Gene Expression and Replication by RNase P Ribozyme.
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ABSTRACT:
SUBMITTER: Liu Y
PROVIDER: S-EPMC10142857 | biostudies-literature | 2023 Apr
REPOSITORIES: biostudies-literature
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Suppressing Kaposi's Sarcoma-Associated Herpesvirus Lytic Gene Expression and Replication by RNase P Ribozyme.
Liu Yujun Y Chen Yuan-Chuan YC Yan Bin B Liu Fenyong F
Molecules (Basel, Switzerland) 20230421 8
Kaposi's sarcoma, an AIDS-defining illness, is caused by Kaposi's sarcoma-associated herpesvirus (KSHV), an oncogenic virus. In this study, we engineered ribozymes derived from ribonuclease P (RNase P) catalytic RNA with targeting against the mRNA encoding KSHV immediate early replication and transcription activator (RTA), which is vital for KSHV gene expression. The functional ribozyme F-RTA efficiently sliced the RTA mRNA sequence in vitro. In cells, KSHV production was suppressed with ribozym ...[more]
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