A simple, dual direct expression plasmid system in prokaryotic and mammalian cells.
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ABSTRACT:
SUBMITTER: Murakami M
PROVIDER: S-EPMC10165797 | biostudies-literature | 2023 May
REPOSITORIES: biostudies-literature
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A simple, dual direct expression plasmid system in prokaryotic and mammalian cells.
Murakami Manabu M Murakami Agnieszka M AM Yonekura Manabu M Miyoshi Ichiro I Itagaki Shirou S Niwa Yasutaka Y
PNAS nexus 20230418 5
We introduce a simple, dual direct cloning plasmid system (pgMAX-II) for gene expression analysis in both prokaryotic (<i>Escherichia coli</i>) and mammalian cells. This system, which uses a prokaryotic expression unit adapted from the pgMAX system and a mammalian promoter, is effective for subcloning using the DNA topoisomerase II toxin CcdB. Given that molecular biological cloning systems broadly rely on <i>E. coli</i> for rapid growth, the proposed concept may have wide applicability beyond m ...[more]
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