Ontology highlight
ABSTRACT:
SUBMITTER: Maze A
PROVIDER: S-EPMC6982536 | biostudies-literature | 2020 Feb
REPOSITORIES: biostudies-literature
Mazé Alain A Benenson Yaakov Y
Nature chemical biology 20191216 2
Augmenting live cells with new signal transduction capabilities is a key objective in genetic engineering and synthetic biology. We showed earlier that two-component signaling pathways could function in mammalian cells, albeit while losing their ligand sensitivity. Here, we show how to transduce small-molecule ligands in a dose-dependent fashion into gene expression in mammalian cells using two-component signaling machinery. First, we engineer mutually complementing truncated mutants of a histid ...[more]