Ontology highlight
ABSTRACT:
SUBMITTER: Zheng C
PROVIDER: S-EPMC10187157 | biostudies-literature | 2023 May
REPOSITORIES: biostudies-literature
Zheng Chen C Weinstein Liana D LD Nguyen Kevin K KK Grewal Abhijeet A Gurevich Eugenia V EV Gurevich Vsevolod V VV
bioRxiv : the preprint server for biology 20230501
Arrestins bind active phosphorylated G protein-coupled receptors (GPCRs). Among the four mammalian subtypes, only arrestin-3 facilitates the activation of JNK3 in cells. In available structures, Lys-295 in the lariat loop of arrestin-3 and its homologue Lys-294 in arrestin-2 directly interact with the activator-attached phosphates. We compared the role of arrestin-3 conformational equilibrium and of Lys-295 in GPCR binding and JNK3 activation. Several mutants with enhanced ability to bind GPCRs ...[more]