Ontology highlight
ABSTRACT:
SUBMITTER: Wu S
PROVIDER: S-EPMC10213282 | biostudies-literature | 2023
REPOSITORIES: biostudies-literature
Wu Shaopeng S Ding Tian T Shao Hongxia H Qian Kun K Ye Jianqiang J Qin Aijian A
Frontiers in veterinary science 20230512
To evaluate the effect of the vaccine and differentiate vaccine from virulent MDV, a new quadruplex real-time PCR assay based on TaqMan probes was developed to differentiate and accurately quantify HVT, CVI988 and virulent MDV-1. The results showed that the limit of detection (LOD) of the new assay was 10 copies with correlation coefficients >0.994 of CVI988, HVT and virulent MDV DNA molecules without cross-reactivity with other avian disease viruses. The intra-assay and inter-assay coefficients ...[more]