Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD.
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ABSTRACT:
SUBMITTER: Leal CA
PROVIDER: S-EPMC3910209 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
Publications
Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD.
Leal C A G CA Carvalho-Castro G A GA Cottorello A C AC Leite R C RC Figueiredo H C P HC
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] 20131115 3
The aims of this study were to standard and optimize a qPCR protocol with FAM-BHQ1 probe, and to compare its sensitivity against TaqMan qPCR and PCR methods to diagnose shrimp WSD. The FAM-BHQ1 qPCR presented higher clinical sensitivity and showed to be a robust alternative to detect WSSV in clinical samples. ...[more]
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