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Antibacterial and Cytocompatible pH-Responsive Peptide Hydrogel.

ABSTRACT: A short peptide, FHHF-11, was designed to change stiffness as a function of pH due to changing degree of protonation of histidines. As pH changes in the physiologically relevant range, G' was measured at 0 Pa (pH 6) and 50,000 Pa (pH 8). This peptide-based hydrogel is antimicrobial and cytocompatible with skin cells (fibroblasts). It was demonstrated that the incorporation of unnatural AzAla tryptophan analog residue improves the antimicrobial properties of the hydrogel. The material developed can have a practical application and be a paradigm shift in the approach to wound treatment, and it will improve healing outcomes for millions of patients each year.

SUBMITTER: Edirisinghe DIU

PROVIDER: S-EPMC10254169 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature

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Antibacterial and Cytocompatible pH-Responsive Peptide Hydrogel.

Edirisinghe Dona Imanga Upamadi DIU D'Souza Areetha A Ramezani Maryam M Carroll Robert J RJ Chicón Quenten Q Muenzel Cheyene L CL Soule Jonathan J Monroe Mary Beth Browning MBB Patteson Alison E AE Makhlynets Olga V OV

Molecules (Basel, Switzerland) 20230527 11

A short peptide, FHHF-11, was designed to change stiffness as a function of pH due to changing degree of protonation of histidines. As pH changes in the physiologically relevant range, G' was measured at 0 Pa (pH 6) and 50,000 Pa (pH 8). This peptide-based hydrogel is antimicrobial and cytocompatible with skin cells (fibroblasts). It was demonstrated that the incorporation of unnatural AzAla tryptophan analog residue improves the antimicrobial properties of the hydrogel. The material developed c ...[more]

PMID: 37298865

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