Dataset Information

Point-of-care applicable metabotyping using biofluid-specific electrospun MetaSAMPs directly amenable to ambient LA-REIMS.

ABSTRACT: In recent years, ambient ionization mass spectrometry (AIMS) including laser ablation rapid evaporation IMS, has enabled direct biofluid metabolome analysis. AIMS procedures are, however, still hampered by both analytical, i.e., matrix effects, and practical, i.e., sample transport stability, drawbacks that impede metabolome coverage. In this study, we aimed at developing biofluid-specific metabolome sampling membranes (MetaSAMPs) that offer a directly applicable and stabilizing substrate for AIMS. Customized rectal, salivary, and urinary MetaSAMPs consisting of electrospun (nano)fibrous membranes of blended hydrophilic (polyvinylpyrrolidone and polyacrylonitrile) and lipophilic (polystyrene) polymers supported metabolite absorption, adsorption, and desorption. Moreover, MetaSAMP demonstrated superior metabolome coverage and transport stability compared to crude biofluid analysis and was successfully validated in two pediatric cohorts (MetaBEAse, n = 234 and OPERA, n = 101). By integrating anthropometric and (patho)physiological with MetaSAMP-AIMS metabolome data, we obtained substantial weight-driven predictions and clinical correlations. In conclusion, MetaSAMP holds great clinical application potential for on-the-spot metabolic health stratification.

SUBMITTER: De Spiegeleer M

PROVIDER: S-EPMC10256167 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature

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Point-of-care applicable metabotyping using biofluid-specific electrospun MetaSAMPs directly amenable to ambient LA-REIMS.

De Spiegeleer Margot M Plekhova Vera V Geltmeyer Jozefien J Schoolaert Ella E Pomian Beata B Singh Varoon V Wijnant Kathleen K De Windt Kimberly K Paukku Volter V De Loof Alexander A Gies Inge I Michels Nathalie N De Henauw Stefaan S De Graeve Marilyn M De Clerck Karen K Vanhaecke Lynn L

Science advances 20230609 23

In recent years, ambient ionization mass spectrometry (AIMS) including laser ablation rapid evaporation IMS, has enabled direct biofluid metabolome analysis. AIMS procedures are, however, still hampered by both analytical, i.e., matrix effects, and practical, i.e., sample transport stability, drawbacks that impede metabolome coverage. In this study, we aimed at developing biofluid-specific metabolome sampling membranes (MetaSAMPs) that offer a directly applicable and stabilizing substrate for AI ...[more]

PMID: 37294759

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Project description:Before pushbroom hyperspectral imaging (HSI) data can be applied in remote sensing applications, it must typically be preprocessed through radiometric correction, atmospheric compensation, geometric correction and spatial resampling procedures. After these preprocessing procedures, HSI data are conventionally given as georeferenced raster images. The raster data model compromises the spatial-spectral integrity of HSI data, leading to suboptimal results in various applications. Inamdar et al. (2021) developed a point cloud data format, the Directly-Georeferenced Hyperspectral Point Cloud (DHPC), that preserves the spatial-spectral integrity of HSI data more effectively than rasters. The DHPC is generated through a data fusion workflow that uses conventional preprocessing protocols with a modification to the digital surface model used in the geometric correction. Even with the additional elevation information, the DHPC is still stored with file sizes up to 13 times smaller than conventional rasters, making it ideal for data distribution. Our article aims to describe the DHPC data fusion workflow from Inamdar et al. (2021), providing all the required tools for its integration in pre-existing processing workflows. This includes a MATLAB script that can be readily applied to carry out the modification that must be made to the digital surface model used in the geometric correction. The MATLAB script first derives the point spread function of the HSI data and then convolves it with the digital surface model input in the geometric correction. By breaking down the MATLAB script and describing its functions, data providers can readily develop their own implementation if necessary. The derived point spread function is also useful for characterizing HSI data, quantifying the contribution of materials to the spectrum from any given pixel as a function of distance from the pixel center. Overall, our work makes the implementation of the DHPC data fusion workflow transparent and approachable for end users and data providers.•Our article describes the Directly-Georeferenced Hyperspectral Point Cloud (DHPC) data fusion workflow, which can be readily implemented with existing processing protocols by modifying the input digital surface model used in the geometric correction.•We provide a MATLAB function that performs the modification to the digital surface model required for the DHPC workflow. This MATLAB script derives the point spread function of the hyperspectral imager and convolves it with the digital surface model so that the elevation data are more spatially consistent with the hyperspectral imaging data as collected.•We highlight the increased effectiveness of the DHPC over conventional raster end products in terms of spatial-spectral data integrity, data storage requirements, hyperspectral imaging application results and site exploration via virtual and augmented reality.

Dataset Information

Point-of-care applicable metabotyping using biofluid-specific electrospun MetaSAMPs directly amenable to ambient LA-REIMS.

Publications

Point-of-care applicable metabotyping using biofluid-specific electrospun MetaSAMPs directly amenable to ambient LA-REIMS.

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