Ontology highlight
ABSTRACT:
SUBMITTER: Maiti A
PROVIDER: S-EPMC10338489 | biostudies-literature | 2023 Jul
REPOSITORIES: biostudies-literature
Maiti Atanu A Buffalo Cosmo Z CZ Saurabh Saumya S Montecinos-Franjola Felipe F Hachey Justin S JS Conlon William J WJ Tran Geraldine N GN Hassan Bakar B Walters Kylie J KJ Drobizhev Mikhail M Moerner W E WE Ghosh Partho P Matsuo Hiroshi H Tsien Roger Y RY Lin John Y JY Rodriguez Erik A EA
Nature communications 20230712 1
The small Ultra-Red Fluorescent Protein (smURFP) represents a new class of fluorescent protein with exceptional photostability and brightness derived from allophycocyanin in a previous directed evolution. Here, we report the smURFP crystal structure to better understand properties and enable further engineering of improved variants. We compare this structure to the structures of allophycocyanin and smURFP mutants to identify the structural origins of the molecular brightness. We then use a struc ...[more]