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Recent Advances in β-Glucosidase Sequence and Structure Engineering: A Brief Review.

ABSTRACT: β-glucosidases (BGLs) play a crucial role in the degradation of lignocellulosic biomass as well as in industrial applications such as pharmaceuticals, foods, and flavors. However, the application of BGLs has been largely hindered by issues such as low enzyme activity, product inhibition, low stability, etc. Many approaches have been developed to engineer BGLs to improve these enzymatic characteristics to facilitate industrial production. In this article, we review the recent advances in BGL engineering in the field, including the efforts from our laboratory. We summarize and discuss the BGL engineering studies according to the targeted functions as well as the specific strategies used for BGL engineering.

SUBMITTER: Ouyang B

PROVIDER: S-EPMC10343665 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature

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Recent Advances in β-Glucosidase Sequence and Structure Engineering: A Brief Review.

Ouyang Bei B Wang Guoping G Zhang Nian N Zuo Jiali J Huang Yunhong Y Zhao Xihua X

Molecules (Basel, Switzerland) 20230625 13

β-glucosidases (BGLs) play a crucial role in the degradation of lignocellulosic biomass as well as in industrial applications such as pharmaceuticals, foods, and flavors. However, the application of BGLs has been largely hindered by issues such as low enzyme activity, product inhibition, low stability, etc. Many approaches have been developed to engineer BGLs to improve these enzymatic characteristics to facilitate industrial production. In this article, we review the recent advances in BGL engi ...[more]

PMID: 37446652

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