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OSpRad: an open-source, low-cost, high-sensitivity spectroradiometer.

ABSTRACT: Spectroradiometry is a vital tool in a wide range of biological, physical, astronomical and medical fields, yet its cost and accessibility are frequent barriers to use. Research into the effects of artificial light at night (ALAN) further compounds these difficulties with requirements for sensitivity to extremely low light levels across the ultraviolet to human-visible spectrum. Here, I present an open-source spectroradiometry (OSpRad) system that meets these design challenges. The system utilises an affordable miniature spectrometer chip (Hamamatsu C12880MA), combined with an automated shutter and cosine-corrector, microprocessor controller, and graphical user interface 'app' that can be used with smartphones or desktop computers. The system has high ultraviolet sensitivity and can measure spectral radiance at 0.001 cd m-2 and irradiance at 0.005 lx, covering the vast majority of real-world night-time light levels. The OSpRad system's low cost and high sensitivity make it well suited to a range of spectrometry and ALAN research.

SUBMITTER: Troscianko J

PROVIDER: S-EPMC10357011 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature

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OSpRad: an open-source, low-cost, high-sensitivity spectroradiometer.

Troscianko Jolyon J

The Journal of experimental biology 20230712 13

Spectroradiometry is a vital tool in a wide range of biological, physical, astronomical and medical fields, yet its cost and accessibility are frequent barriers to use. Research into the effects of artificial light at night (ALAN) further compounds these difficulties with requirements for sensitivity to extremely low light levels across the ultraviolet to human-visible spectrum. Here, I present an open-source spectroradiometry (OSpRad) system that meets these design challenges. The system utilis ...[more]

PMID: 37334657

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Project description:BackgroundRemote monitoring of plants using hyperspectral imaging has become an important tool for the study of plant growth, development, and physiology. Many applications are oriented towards use in field environments to enable non-destructive analysis of crop responses due to factors such as drought, nutrient deficiency, and disease, e.g., using tram, drone, or airplane mounted instruments. The field setting introduces a wide range of uncontrolled environmental variables that make validation and interpretation of spectral responses challenging, and as such lab- and greenhouse-deployed systems for plant studies and phenotyping are of increasing interest. In this study, we have designed and developed an open-source, hyperspectral reflectance-based imaging system for lab-based plant experiments: the HyperScanner. The reliability and accuracy of HyperScanner were validated using drought and salt stress experiments with Arabidopsis thaliana.ResultsA robust, scalable, and reliable system was created. The system was built using open-sourced parts, and all custom parts, operational methods, and data have been made publicly available in order to maintain the open-source aim of HyperScanner. The gathered reflectance images showed changes in narrowband red and infrared reflectance spectra for each of the stress tests that was evident prior to other visual physiological responses and exhibited congruence with measurements using full-range contact spectrometers.ConclusionsHyperScanner offers the potential for reliable and inexpensive laboratory hyperspectral imaging systems. HyperScanner was able to quickly collect accurate reflectance curves on a variety of plant stress experiments. The resulting images showed spectral differences in plants shortly after application of a treatment but before visual manifestation. HyperScanner increases the capacity for spectroscopic and imaging-based analytical tools by providing more access to hyperspectral analyses in the laboratory setting.

Dataset Information

OSpRad: an open-source, low-cost, high-sensitivity spectroradiometer.

Publications

OSpRad: an open-source, low-cost, high-sensitivity spectroradiometer.

Similar Datasets

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