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Prospecting for Zoonotic Pathogens by Using Targeted DNA Enrichment.

ABSTRACT: More than 60 zoonoses are linked to small mammals, including some of the most devastating pathogens in human history. Millions of museum-archived tissues are available to understand natural history of those pathogens. Our goal was to maximize the value of museum collections for pathogen-based research by using targeted sequence capture. We generated a probe panel that includes 39,916 80-bp RNA probes targeting 32 pathogen groups, including bacteria, helminths, fungi, and protozoans. Laboratory-generated, mock-control samples showed that we are capable of enriching targeted loci from pathogen DNA 2,882‒6,746-fold. We identified bacterial species in museum-archived samples, including Bartonella, a known human zoonosis. These results showed that probe-based enrichment of pathogens is a highly customizable and efficient method for identifying pathogens from museum-archived tissues.

SUBMITTER: Enabulele EE

PROVIDER: S-EPMC10370864 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature

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Prospecting for Zoonotic Pathogens by Using Targeted DNA Enrichment.

Enabulele Egie E EE Le Clec'h Winka W Roberts Emma K EK Thompson Cody W CW McDonough Molly M MM Ferguson Adam W AW Bradley Robert D RD Anderson Timothy J C TJC Platt Roy N RN

Emerging infectious diseases 20230801 8

More than 60 zoonoses are linked to small mammals, including some of the most devastating pathogens in human history. Millions of museum-archived tissues are available to understand natural history of those pathogens. Our goal was to maximize the value of museum collections for pathogen-based research by using targeted sequence capture. We generated a probe panel that includes 39,916 80-bp RNA probes targeting 32 pathogen groups, including bacteria, helminths, fungi, and protozoans. Laboratory-g ...[more]

PMID: 37486179

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