Ontology highlight
ABSTRACT:
SUBMITTER: Shearer AE
PROVIDER: S-EPMC3561655 | biostudies-other | 2012 May
REPOSITORIES: biostudies-other
Shearer Aiden Eliot AE Hildebrand Michael S MS Smith Richard J H RJ
BMC biotechnology 20120504
<h4>Background</h4>Solution-based targeted genomic enrichment (TGE) protocols permit selective sequencing of genomic regions of interest on a massively parallel scale. These protocols could be improved by: 1) modifying or eliminating time consuming steps; 2) increasing yield to reduce input DNA and excessive PCR cycling; and 3) enhancing reproducible.<h4>Results</h4>We developed a solution-based TGE method for downstream Illumina sequencing in a non-automated workflow, adding standard Illumina b ...[more]