Dataset Information

Impact of the fungal pathogen Fusarium oxysporum on the taxonomic and functional diversity of the common bean root microbiome.

ABSTRACT:

Background

Plants rely on their root microbiome as the first line of defense against soil-borne fungal pathogens. The abundance and activities of beneficial root microbial taxa at the time prior to and during fungal infection are key to their protective success. If and how invading fungal root pathogens can disrupt microbiome assembly and gene expression is still largely unknown. Here, we investigated the impact of the fungal pathogen Fusarium oxysporum (fox) on the assembly of rhizosphere and endosphere microbiomes of a fox-susceptible and fox-resistant common bean cultivar.

Results

Integration of 16S-amplicon, shotgun metagenome as well as metatranscriptome sequencing with community ecology analysis showed that fox infections significantly changed the composition and gene expression of the root microbiome in a cultivar-dependent manner. More specifically, fox infection led to increased microbial diversity, network complexity, and a higher proportion of the genera Flavobacterium, Bacillus, and Dyadobacter in the rhizosphere of the fox-resistant cultivar compared to the fox-susceptible cultivar. In the endosphere, root infection also led to changes in community assembly, with a higher abundance of the genera Sinorhizobium and Ensifer in the fox-resistant cultivar. Metagenome and metatranscriptome analyses further revealed the enrichment of terpene biosynthesis genes with a potential role in pathogen suppression in the fox-resistant cultivar upon fungal pathogen invasion.

Conclusion

Collectively, these results revealed a cultivar-dependent enrichment of specific bacterial genera and the activation of putative disease-suppressive functions in the rhizosphere and endosphere microbiome of common bean under siege.

SUBMITTER: Mendes LW

PROVIDER: S-EPMC10401788 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature

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Publications

Impact of the fungal pathogen Fusarium oxysporum on the taxonomic and functional diversity of the common bean root microbiome.

Mendes Lucas William LW Raaijmakers Jos M JM de Hollander Mattias M Sepo Edis E Gómez Expósito Ruth R Chiorato Alisson Fernando AF Mendes Rodrigo R Tsai Siu Mui SM Carrión Victor J VJ

Environmental microbiome 20230803 1

<h4>Background</h4>Plants rely on their root microbiome as the first line of defense against soil-borne fungal pathogens. The abundance and activities of beneficial root microbial taxa at the time prior to and during fungal infection are key to their protective success. If and how invading fungal root pathogens can disrupt microbiome assembly and gene expression is still largely unknown. Here, we investigated the impact of the fungal pathogen Fusarium oxysporum (fox) on the assembly of rhizosphe ...[more]

PMID: 37537681

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Project description:Beyond interacting with neighboring plants, crop performance is affected by the microbiome that includes pathogens and mutualists. While the importance of plant-plant interactions in explaining overyielding in intercropping is well known, the role of the microbiome, in particular how the presence of microbes from heterospecific crop species inhibit pathogens of the focal plants in affecting yield remains hardly explored. Here we performed both field samplings and pot experiments to investigate the microbial interactions in the maize/faba bean intercropping system, with the focus on the inhibition of Fusarium oxysporum in faba bean plants. Long-term field measurements show that maize/faba bean intercropping increased crop yield, reduced the gene copies of F. oxysporum by 30-84% and increased bacterial richness and Shannon index compared to monocropping. Bacterial networks in intercropping were more stable with more hub nodes than the respective monocultures. Furthermore, the observed changes of whole microbial communities were aligned with differences in the number of siderophore-producing rhizobacteria in maize and pathogen abundances in faba bean. Maize possessed 71% more siderophore-producing rhizobacteria and 33% more synthetases genes abundance of nonribosomal peptides, especially pyochelin, relative to faba bean. This was further evidenced by the increased numbers of siderophore-producing bacteria and decreased gene copies of F. oxysporum in the rhizosphere of intercropped faba bean. Four bacteria (Pseudomonas spp. B004 and B021, Bacillus spp. B005 and B208) from 95 isolates antagonized F. oxysporum f. sp. fabae. In particular, B005, which represented a hub node in the networks, showed particularly high siderophore-producing capabilities. Intercropping increased overall bacterial diversity and network complexity and the abundance of siderophore-producing bacteria, leading to facilitated pathogen suppression and increased resistance of faba bean to F. oxysporum. This study has great agronomic implications as microorganisms might be specifically targeted to optimize intercropping practices in the future.

Dataset Information

Impact of the fungal pathogen Fusarium oxysporum on the taxonomic and functional diversity of the common bean root microbiome.

Background

Results

Conclusion

Publications

Impact of the fungal pathogen Fusarium oxysporum on the taxonomic and functional diversity of the common bean root microbiome.

Similar Datasets

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