Dataset Information

Decoding the spermatogonial stem cell niche under physiological and recovery conditions in adult mice and humans.

ABSTRACT: The intricate interaction between spermatogonial stem cell (SSC) and testicular niche is essential for maintaining SSC homeostasis; however, this interaction remains largely uncharacterized. In this study, to characterize the underlying signaling pathways and related paracrine factors, we delineated the intercellular interactions between SSC and niche cell in both adult mice and humans under physiological conditions and dissected the niche-derived regulation of SSC maintenance under recovery conditions, thus uncovering the essential role of C-C motif chemokine ligand 24 and insulin-like growth factor binding protein 7 in SSC maintenance. We also established the clinical relevance of specific paracrine factors in human fertility. Collectively, our work on decoding the adult SSC niche serves as a valuable reference for future studies on the aetiology, diagnosis, and treatment of male infertility.

SUBMITTER: Jin C

PROVIDER: S-EPMC10403211 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Decoding the spermatogonial stem cell niche under physiological and recovery conditions in adult mice and humans.

Jin Cheng C Wang Zhipeng Z Li Pengyu P Tang Jielin J Jiao Tao T Li Yiran Y Ou Jinhuan J Zou Dingfeng D Li Mengzhen M Mang Xinyu X Liu Jun J Ma Yanni Y Wu Xiaolong X Shi Jie J Chen Shitao S He Manman M Lu Yan Y Zhang Ning N Miao Shiying S Sun Fei F Wang Linfang L Li Kai K Yu Jia J Song Wei W

Science advances 20230804 31

The intricate interaction between spermatogonial stem cell (SSC) and testicular niche is essential for maintaining SSC homeostasis; however, this interaction remains largely uncharacterized. In this study, to characterize the underlying signaling pathways and related paracrine factors, we delineated the intercellular interactions between SSC and niche cell in both adult mice and humans under physiological conditions and dissected the niche-derived regulation of SSC maintenance under recovery con ...[more]

PMID: 37540753

Similar Datasets

Project description:Study hypothesisAre active ovarian germ stem cells present in postnatal mouse ovaries under physiological conditions?Study findingActive ovarian germ stem cells exist and function in adult mouse ovaries under physiological conditions.What is known alreadyIn vitro studies suggested the existence of germ stem cells in postnatal ovaries of mouse, pig and human. However, in vivo studies provided evidence against the existence of active germ stem cells in postnatal mouse ovaries. Thus, it remains controversial whether such germ stem cells really exist and function in vivo in postnatal mammalian ovaries.Study design, samples/materials, methodsOctamer-binding transcription factor 4 (Oct4)-MerCreMer transgenic mice were crossed with R26R-enhanced yellow fluorescent protein (EYFP) mice to establish a tamoxifen-inducible tracing system so that Oct4-expressing potential ovarian germ stem cells in young adult mice (5-6 weeks old) can be labeled with EYFP. The germ cell activities of DNA replication, mitotic division, entry into meiosis and progression to primordial follicle stage were investigated by means of immunofluorescent staining of ovarian tissues collected at different time points post-tamoxifen injection (1 day, 3 days, 2 months and 4 months). Meiosis entry and primordial follicle formation were also measured by EYFP-labeled single-cell RT-PCR. Germ cell proliferation and mitotic division were examined through 5-bromodeoxyuridine triphosphate incorporation assay. At each time point, ovaries from two to three animals were used for each set of experiment.Main results and the role of chanceBy labeling the Oct4-expressing small germ cells and tracing their fates for up to 4 months, we observed persistent meiosis entry and primordial follicle replenishment. Furthermore, we captured the transient processes of mitotic DNA replication as well as mitotic division of the marked germ cells at various time periods after tracing. These lines of evidence unambiguously support the presence of active germ stem cells in postnatal ovaries and their function in replenishing primordial follicle pool under physiological conditions. Moreover, we pointed out that Oct4(+) deleted in azoospermia-like (Dazl)(-) but not Oct4(+)Dazl(+) or Oct4(+) DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 4 (Ddx4)(+) cells contain a population of germ stem cells in mouse ovary.Limitations, reasons for cautionThis study was conducted in mice. Whether or not the results are applicable to human remain unclear. The future work should aim at identifying the specific ovarian germ stem cell marker and evaluating the significance of these stem cells to normal ovarian function.Wider implications of the findingsClarifying the existence of active germ stem cells and their functional significance in postnatal mammalian ovaries could provide new insights in understanding the mechanism of ovarian aging and failure.Large scale dataNot applicable.Study funding/competing interestsThis work was supported by the National Key Basic Research Program of China (grant number 2012CBA01300) and the National Natural Science Foundation of China to P.Z. (31571484). No competing interests are reported.

Dataset Information

Decoding the spermatogonial stem cell niche under physiological and recovery conditions in adult mice and humans.

Publications

Decoding the spermatogonial stem cell niche under physiological and recovery conditions in adult mice and humans.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets