Dataset Information

Complete mitochondrial genomes of the slugs Deroceraslaeve (Agriolimacidae) and Ambigolimaxvalentianus (Limacidae) provide insights into the phylogeny of Stylommatophora (Mollusca, Gastropoda).

ABSTRACT: In this study, we sequenced two complete mitogenomes from Deroceraslaeve and Ambigolimaxvalentianus. The mitogenome of Ambigolimaxvalentianus represented the first such data from the family Limacidae. The lengths of the mitogenomes of Deroceraslaeve and Ambigolimaxvalentianus were 14,773 bp and 15,195 bp, respectively. The entire set of 37 mitochondrial genes were identified for both mitogenomes. Compared with the mitogenome of Achatinafulica, the trnP_trnA tRNA cluster was rearranged in both Deroceraslaeve and Ambigolimaxvalentianus. The secondary structures of tRNA and rRNA genes for the two species were predicted. Phylogenetic analyses based on amino acid sequences supported (1) monophyly of Stylommatophora, (2) division of Stylommatophora into the 'achatinoid' clade (i.e., the suborder Achatinina) and the 'non-achatinoid' clade (i.e., the suborder Helicina), (3) placement of the Orthurethra in the 'non-achatinoid' clade, and (4) monophyly of each of the superfamilies Helicoidea, Urocoptoidea, Succineoidea, Arionoidea, Pupilloidea and Limacoidea. The exemplars of Helicidae, Philomycidae and Achatinellidae displayed many more mitochondrial gene rearrangements than other species of Stylommatophora.

SUBMITTER: Zhao T

PROVIDER: S-EPMC10407649 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Complete mitochondrial genomes of the slugs Deroceraslaeve (Agriolimacidae) and Ambigolimaxvalentianus (Limacidae) provide insights into the phylogeny of Stylommatophora (Mollusca, Gastropoda).

Zhao Te T Song Nan N Lin Xingyu X Zhang Yang Y

ZooKeys 20230731

In this study, we sequenced two complete mitogenomes from Deroceraslaeve and Ambigolimaxvalentianus. The mitogenome of Ambigolimaxvalentianus represented the first such data from the family Limacidae. The lengths of the mitogenomes of Deroceraslaeve and Ambigolimaxvalentianus were 14,773 bp and 15,195 bp, respectively. The entire set of 37 mitochondrial genes were identified for both mitogenomes. Compared with the mitogenome of Achatinafulica, the trn ...[more]

PMID: 37560262

Similar Datasets

Project description:BackgroundReconstructing the higher relationships of pulmonate gastropods has been difficult. The use of morphology is problematic due to high homoplasy. Molecular studies have suffered from low taxon sampling. Forty-eight complete mitochondrial genomes are available for gastropods, ten of which are pulmonates. Here are presented the new complete mitochondrial genomes of the ten following species of pulmonates: Salinator rhamphidia (Amphiboloidea); Auriculinella bidentata, Myosotella myosotis, Ovatella vulcani, and Pedipes pedipes (Ellobiidae); Peronia peronii (Onchidiidae); Siphonaria gigas (Siphonariidae); Succinea putris (Stylommatophora); Trimusculus reticulatus (Trimusculidae); and Rhopalocaulis grandidieri (Veronicellidae). Also, 94 new pulmonate-specific primers across the entire mitochondrial genome are provided, which were designed for amplifying entire mitochondrial genomes through short reactions and closing gaps after shotgun sequencing.ResultsThe structural features of the 10 new mitochondrial genomes are provided. All genomes share similar gene orders. Phylogenetic analyses were performed including the 10 new genomes and 17 genomes from Genbank (outgroups, opisthobranchs, and other pulmonates). Bayesian Inference and Maximum Likelihood analyses, based on the concatenated amino-acid sequences of the 13 protein-coding genes, produced the same topology. The pulmonates are paraphyletic and basal to the opisthobranchs that are monophyletic at the tip of the tree. Siphonaria, traditionally regarded as a basal pulmonate, is nested within opisthobranchs. Pyramidella, traditionally regarded as a basal (non-euthyneuran) heterobranch, is nested within pulmonates. Several hypotheses are rejected, such as the Systellommatophora, Geophila, and Eupulmonata. The Ellobiidae is polyphyletic, but the false limpet Trimusculus reticulatus is closely related to some ellobiids.ConclusionsDespite recent efforts for increasing the taxon sampling in euthyneuran (opisthobranchs and pulmonates) molecular phylogenies, several of the deeper nodes are still uncertain, because of low support values as well as some incongruence between analyses based on complete mitochondrial genomes and those based on individual genes (18S, 28S, 16S, CO1). Additional complete genomes are needed for pulmonates (especially for Williamia, Otina, and Smeagol), as well as basal heterobranchs closely related to euthyneurans. Increasing the number of markers for gastropod (and more broadly mollusk) phylogenetics also is necessary in order to resolve some of the deeper nodes -although clearly not an easy task. Step by step, however, new relationships are being unveiled, such as the close relationships between the false limpet Trimusculus and ellobiids, the nesting of pyramidelloids within pulmonates, and the close relationships of Siphonaria to sacoglossan opisthobranchs. The additional genomes presented here show that some species share an identical mitochondrial gene order due to convergence.

Project description:BackgroundThe genus Zingiber of the Zingiberaceae is distributed in tropical, subtropical, and in Far East Asia. This genus contains about 100-150 species, with many species valued as important agricultural, medicinal and horticultural resources. However, genomic resources and suitable molecular markers for species identification are currently sparse.ResultsWe conducted comparative genomics and phylogenetic analyses on Zingiber species. The Zingiber chloroplast genome (size range 162,507-163,711 bp) possess typical quadripartite structures that consist of a large single copy (LSC, 86,986-88,200 bp), a small single copy (SSC, 15,498-15,891 bp) and a pair of inverted repeats (IRs, 29,765-29,934 bp). The genomes contain 113 unique genes, including 79 protein coding genes, 30 tRNA and 4 rRNA genes. The genome structures, gene contents, amino acid frequencies, codon usage patterns, RNA editing sites, simple sequence repeats and long repeats are conservative in the genomes of Zingiber. The analysis of sequence divergence indicates that the following genes undergo positive selection (ccsA, ndhA, ndhB, petD, psbA, psbB, psbC, rbcL, rpl12, rpl20, rpl23, rpl33, rpoC2, rps7, rps12 and ycf3). Eight highly variable regions are identified including seven intergenic regions (petA-pabJ, rbcL-accD, rpl32-trnL-UAG, rps16-trnQ-UUG, trnC-GCA-psbM, psbC-trnS-UGA and ndhF-rpl32) and one genic regions (ycf1). The phylogenetic analysis revealed that the sect. Zingiber was sister to sect. Cryptanthium rather than sect. Pleuranthesis.ConclusionsThis study reports 14 complete chloroplast genomes of Zingiber species. Overall, this study provided a solid backbone phylogeny of Zingiber. The polymorphisms we have uncovered in the sequencing of the genome offer a rare possibility (for Zingiber) of the generation of DNA markers. These results provide a foundation for future studies that seek to understand the molecular evolutionary dynamics or individual population variation in the genus Zingiber.

Dataset Information

Complete mitochondrial genomes of the slugs Deroceraslaeve (Agriolimacidae) and Ambigolimaxvalentianus (Limacidae) provide insights into the phylogeny of Stylommatophora (Mollusca, Gastropoda).

Publications

Complete mitochondrial genomes of the slugs <i>Deroceraslaeve</i> (Agriolimacidae) and <i>Ambigolimaxvalentianus</i> (Limacidae) provide insights into the phylogeny of Stylommatophora (Mollusca, Gastropoda).

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Dataset Information

Complete mitochondrial genomes of the slugs Deroceraslaeve (Agriolimacidae) and Ambigolimaxvalentianus (Limacidae) provide insights into the phylogeny of Stylommatophora (Mollusca, Gastropoda).

Publications

﻿Complete mitochondrial genomes of the slugs <i>Deroceraslaeve</i> (Agriolimacidae) and <i>Ambigolimaxvalentianus</i> (Limacidae) provide insights into the phylogeny of Stylommatophora (Mollusca, Gastropoda).

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Complete mitochondrial genomes of the slugs <i>Deroceraslaeve</i> (Agriolimacidae) and <i>Ambigolimaxvalentianus</i> (Limacidae) provide insights into the phylogeny of Stylommatophora (Mollusca, Gastropoda).