Dataset Information

The complete mitogenome of Helixpomatia and the basal phylogeny of Helicinae (Gastropoda, Stylommatophora, Helicidae).

ABSTRACT: A complete mitochondrial genome of the Roman snail Helixpomatia Linnaeus, 1758 has been sequenced. The length and gene order correspond to that of other available helicid mitogenomes. We used the mitogenome sequence to reappraise the relationships among the four presumed principal groups of the helicid subfamily Helicinae. The results support the idea that the subfamily is divided between two western Palaearctic diversification centres: Iberian Peninsula and western Maghreb in the west, and Anatolia, the Aegean and Caucasus in the east. One group, the tribe Helicini, diversified in the east and the remaining three currently recognised tribes in the west. However, the exact relationships among lineages of the non-Helicini tribes could not be resolved.

SUBMITTER: Orabek O

PROVIDER: S-EPMC6472302 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Publications

The complete mitogenome of <i>Helixpomatia</i> and the basal phylogeny of Helicinae (Gastropoda, Stylommatophora, Helicidae).

Orábek Ondřej O Petrusek Adam A Rovatsos Michail M

ZooKeys 20190305

A complete mitochondrial genome of the Roman snail <i>Helixpomatia</i> Linnaeus, 1758 has been sequenced. The length and gene order correspond to that of other available helicid mitogenomes. We used the mitogenome sequence to reappraise the relationships among the four presumed principal groups of the helicid subfamily Helicinae. The results support the idea that the subfamily is divided between two western Palaearctic diversification centres: Iberian Peninsula and western Maghreb in the west, a ...[more]

PMID: 31114424

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Project description:Stylommatophora is one of the most speciose orders of Gastropoda, including terrestrial snails and slugs, some of which are economically important as human food, agricultural pests, vectors of parasites or due to invasiveness. Despite their great diversity and relevance, the internal phylogeny of Stylommatophora has been debated. To date, only 34 stylommatophoran mitogenomes were sequenced. Here, the complete mitogenome of an invasive pest slug, Arion vulgaris Moquin-Tandon, 1855 (Stylommatophora: Arionidae), was sequenced using next generation sequencing, analysed and compared with other stylommatophorans. The mitogenome of A. vulgaris measures 14,547 bp and contains 13 protein-coding, two rRNA, 22 tRNA genes, and one control region, with an A + T content of 70.20%. All protein coding genes (PCGs) are initiated with ATN codons except for COX1, ND5 and ATP8 and all are ended with TAR or T-stop codons. All tRNAs were folded into a clover-leaf secondary structure except for trnC and trnS1 (AGN). Phylogenetic analyses confirmed the position of A. vulgaris within the superfamily Arionoidea, recovered a sister group relationship between Arionoidea and Orthalicoidea, and supported monophyly of all currently recognized superfamilies within Stylommatophora except for the superfamily Helicoidea. Initial diversification time of the Stylommatophora was estimated as 138.55 million years ago corresponding to Early Cretaceous. The divergence time of A. vulgaris and Arion rufus (Linnaeus, 1758) was estimated as 15.24 million years ago corresponding to one of Earth's most recent, global warming events, the Mid-Miocene Climatic Optimum. Furthermore, selection analyses were performed to investigate the role of different selective forces shaping stylommatophoran mitogenomes. Although purifying selection is the predominant selective force shaping stylommatophoran mitogenomes, six genes (ATP8, COX1, COX3, ND3, ND4 and ND6) detected by the branch-specific aBSREL approach and three genes (ATP8, CYTB and ND4L) detected by codon-based BEB, FUBAR and MEME approaches were exposed to diversifying selection. The positively selected substitutions at the mitochondrial PCGs of stylommatophoran species seems to be adaptive to environmental conditions and affecting mitochondrial ATP production or protection from reactive oxygen species effects. Comparative analysis of stylommatophoran mitogenome rearrangements using MLGO revealed conservatism in Stylommatophora; exceptions refer to potential apomorphies for several clades including rearranged orders of trnW-trnY and of trnE-trnQ-rrnS-trnM-trnL2-ATP8-trnN-ATP6-trnR clusters for the genus Arion. Generally, tRNA genes tend to be rearranged and tandem duplication random loss, transitions and inversions are the most basic mechanisms shaping stylommatophoran mitogenomes.

Project description:BACKGROUND: This study describes how the complete mitogenome of a terrestrial snail, Cylindrus obtusus (Draparnaud, 1805) was sequenced without PCRs from a collection specimen that had been in 70% ethanol for 8 years. The mitogenome was obtained with Illumina GAIIx shot gun sequencing. Although the used specimen was collected relatively recently and kept in a DNA-friendly preservative (not formalin as frequently used with old museum specimens), we believe that the exclusion of PCRs as facilitated by NGS (Next Generation Sequencing) removes a great obstacle in DNA sequencing of collection specimens. A brief comparison is made between our Illumina GAIIx approach and a similar study that made use of the Roche 454-FLX platform. RESULTS: The mtDNA sequence of C. obtusus is 14,610 bases in length (about 0.5 kb larger than other stylommatophoran mitogenomes reported hitherto) and contains the 37 genes (13 protein coding genes, two rRNAs and 22 tRNAs) typical for metazoans. Except for a swap between the position of tRNA-Pro and tRNA-Ala, the gene arrangement of C. obtusus is identical to that reported for Cepaea nemoralis. The 'aberrant' rearrangement of tRNA-Thr and COIII compared to that of other Sigmurethra (and the majority of gastropods), is not unique for C. nemoralis (subfamily Helicinae), but is also shown to occur in C. obtusus (subfamily Ariantinae) and might be a synapomorphy for the family Helicidae. CONCLUSIONS: Natural history collections potentially harbor a wealth of information for the field of evolutionary genetics, but it can be difficult to amplify DNA from such specimens (due to DNA degradation for instance). Because NGS techniques do not rely on primer-directed amplification (PCR) and allow DNA to be fragmented (DNA gets sheared during library preparation), NGS could be a valuable tool for retrieving DNA sequence data from such specimens. A comparison between Illumina GAIIx and the Roche 454 platform suggests that the former might be more suited for de novo sequencing of mitogenomes.

Dataset Information

The complete mitogenome of Helixpomatia and the basal phylogeny of Helicinae (Gastropoda, Stylommatophora, Helicidae).

Publications

The complete mitogenome of <i>Helixpomatia</i> and the basal phylogeny of Helicinae (Gastropoda, Stylommatophora, Helicidae).

Similar Datasets

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