Design of 5' untranslated sequences in retroviral vectors developed for medical use.
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ABSTRACT:
SUBMITTER: Hildinger M
PROVIDER: S-EPMC104187 | biostudies-literature | 1999 May
REPOSITORIES: biostudies-literature
Publications
Design of 5' untranslated sequences in retroviral vectors developed for medical use.
Hildinger M M Abel K L KL Ostertag W W Baum C C
Journal of virology 19990501 5
Utilizing genetic modules of simple retroviruses, we have developed a novel generation of gene transfer vectors with improved therapeutic potential. In the 5' untranslated "leader" sequences, all AUG codons which may aberrantly initiate translation and all viral coding sequences were removed. Thus, the probability of expressing unwanted peptides and the potential for homologous recombination with retroviral genes were largely reduced, and the cloning capacity was increased. The transgene was ins ...[more]
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