Unknown

Dataset Information

0

IS1245 restriction fragment length polymorphism typing of Mycobacterium avium isolates: proposal for standardization.

ABSTRACT: Mycobacterium avium has become a major human pathogen, primarily due to the emergence of the AIDS epidemic. Restriction fragment length polymorphism (RFLP) typing, using insertion sequence IS1245 as a probe, provides a powerful tool in the molecular epidemiology of M. avium-related infections and will facilitate well-founded studies into the sources of M. avium infections in animal and environmental reservoirs. The standardization of this technique allows computerization of IS1245 RFLP patterns for comparison on a local level and the establishment of M. avium DNA fingerprint databases for interlaboratory comparison. Moreover, by combining international DNA typing results of M. avium complex isolates from a broad spectrum of sources, long-lasting questions on the epidemiology of this major agent of mycobacterial infections will be answered.

SUBMITTER: van Soolingen D 

PROVIDER: S-EPMC105111 | biostudies-literature | 1998 Oct

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

IS1245 restriction fragment length polymorphism typing of Mycobacterium avium isolates: proposal for standardization.

van Soolingen D D   Bauer J J   Ritacco V V   Leão S C SC   Pavlik I I   Vincent V V   Rastogi N N   Gori A A   Bodmer T T   Garzelli C C   Garcia M J MJ  

Journal of clinical microbiology 19981001 10

Mycobacterium avium has become a major human pathogen, primarily due to the emergence of the AIDS epidemic. Restriction fragment length polymorphism (RFLP) typing, using insertion sequence IS1245 as a probe, provides a powerful tool in the molecular epidemiology of M. avium-related infections and will facilitate well-founded studies into the sources of M. avium infections in animal and environmental reservoirs. The standardization of this technique allows computerization of IS1245 RFLP patterns  ...[more]

Similar Datasets

Unknown New variable-number tandem-repeat markers for typing Mycobacterium avium subsp. paratuberculosis and M. avium strains: comparison with IS900 and IS1245 restriction fragment length polymorphism typing.
| S-EPMC1951273 | biostudies-literature
Unknown Comparison of a variable-number tandem-repeat (VNTR) method for typing Mycobacterium avium with mycobacterial interspersed repetitive-unit-VNTR and IS1245 restriction fragment length polymorphism typing.
| S-EPMC2708485 | biostudies-literature
Unknown Comparison of variable-number tandem-repeat markers typing and IS1245 restriction fragment length polymorphism fingerprinting of Mycobacterium avium subsp. hominissuis from human and porcine origins.
| S-EPMC2845136 | biostudies-literature
Unknown Use of restriction fragment length polymorphism as a genetic marker for typing Mycobacterium avium strains.
| S-EPMC228175 | biostudies-other
Unknown Typing of Mycobacterium avium subspecies paratuberculosis isolates from Newfoundland using fragment analysis.
| S-EPMC4415927 | biostudies-literature
Unknown Restriction fragment length polymorphism analysis of clinical isolates of Mycobacterium haemophilum.
| S-EPMC263787 | biostudies-other
Clinical Molecular Characterization of Blastocystis Isolates From Human by Restriction Fragment Length Polymorphism (RFLP) Analysis
| 2751436 | ecrin-mdr-crc
Unknown Identification of a novel DNA probe for strain typing Mycobacterium bovis by restriction fragment length polymorphism analysis.
| S-EPMC86571 | biostudies-literature
Unknown Distribution of IS1311 and IS1245 in Mycobacterium avium subspecies revisited.
| S-EPMC1153774 | biostudies-literature
Unknown Determination of genotypic diversity of Mycobacterium avium subspecies from human and animal origins by mycobacterial interspersed repetitive-unit-variable-number tandem-repeat and IS1311 restriction fragment length polymorphism typing methods.
| S-EPMC2849548 | biostudies-literature