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Use of restriction fragment length polymorphism as a genetic marker for typing Mycobacterium avium strains.

ABSTRACT: Restriction fragment length polymorphism (RFLP) was used to study 75 clinical isolates identified as Mycobacterium avium. Two repetitive insertion sequences, IS1311 and IS900, were used as DNA probes. Although less than 25% of isolates showed RFLP patterns with IS900, all strains gave banding patterns with IS1311. M. avium strains isolated from patients with AIDS exhibited marked polymorphism with both probes.

SUBMITTER: Roiz MP

PROVIDER: S-EPMC228175 | biostudies-other | 1995 May

REPOSITORIES: biostudies-other

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Use of restriction fragment length polymorphism as a genetic marker for typing Mycobacterium avium strains.

Roiz M P MP Palenque E E Guerrero C C Garcia M J MJ

Journal of clinical microbiology 19950501 5

Restriction fragment length polymorphism (RFLP) was used to study 75 clinical isolates identified as Mycobacterium avium. Two repetitive insertion sequences, IS1311 and IS900, were used as DNA probes. Although less than 25% of isolates showed RFLP patterns with IS900, all strains gave banding patterns with IS1311. M. avium strains isolated from patients with AIDS exhibited marked polymorphism with both probes. ...[more]

PMID: 7615764

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