Efficient production of CRISPR/Cas9 gene knockouts in the male/female nematode Caenorhabditis nigoni.
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ABSTRACT:
SUBMITTER: Harbin JP
PROVIDER: S-EPMC10512056 | biostudies-literature | 2023
REPOSITORIES: biostudies-literature
Publications
Efficient production of CRISPR/Cas9 gene knockouts in the male/female nematode <i>Caenorhabditis nigoni</i>.
Harbin Jonathan P JP Ellis Ronald E RE
microPublication biology 20230906
Although nematode genetics was founded on the use of hermaphrodite genetics for studying animal development and behavior, there is a growing need to extend this work to male/female species. One of the most promising species is <i>C. nigoni,</i> because it is so closely related to the model hermaphroditic <i>C. briggsae.</i> We present methods for using CRISPR/Cas9 gene editing to create mutations, and techniques for balancing, maintaining and studying these mutations. ...[more]
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