Efficient gene knockout in goats using CRISPR/Cas9 system.
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ABSTRACT:
SUBMITTER: Ni W
PROVIDER: S-EPMC4154755 | biostudies-literature | 2014
REPOSITORIES: biostudies-literature
Publications
Efficient gene knockout in goats using CRISPR/Cas9 system.
Ni Wei W Qiao Jun J Hu Shengwei S Zhao Xinxia X Regouski Misha M Yang Min M Polejaeva Irina A IA Chen Chuangfu C
PloS one 20140904 9
The CRISPR/Cas9 system has been adapted as an efficient genome editing tool in laboratory animals such as mice, rats, zebrafish and pigs. Here, we report that CRISPR/Cas9 mediated approach can efficiently induce monoallelic and biallelic gene knockout in goat primary fibroblasts. Four genes were disrupted simultaneously in goat fibroblasts by CRISPR/Cas9-mediated genome editing. The single-gene knockout fibroblasts were successfully used for somatic cell nuclear transfer (SCNT) and resulted in l ...[more]
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