Ontology highlight
ABSTRACT:
SUBMITTER: Tong L
PROVIDER: S-EPMC10518705 | biostudies-literature | 2023 Oct
REPOSITORIES: biostudies-literature
Tong Lei L Han Shanshan S Xue Yao Y Chen Minggang M Chen Fuyi F Ke Wei W Shu Yousheng Y Ding Ning N Bewersdorf Joerg J Zhou Z Jimmy ZJ Yuan Peng P Grutzendler Jaime J
iScience 20230909 10
Optogenetic manipulation with single-cell resolution can be achieved by two-photon excitation. However, this frequently requires relatively high laser powers. Here, we developed a novel strategy that can improve the efficiency of current two-photon stimulation technologies by positioning fluorescent proteins or small fluorescent molecules with high two-photon cross-sections in the vicinity of opsins. This generates a highly localized source of endogenous single-photon illumination that can be ta ...[more]