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Initial characterization of an iron superoxide dismutase from Thermobifida fusca.


ABSTRACT: Superoxide dismutases (SODs) are enzymes that catalyze the dismutation of the superoxide radical anion into O2 and H2O2 in a two-step reaction. They are ubiquitous to all forms of life and four different types of metal centers are detected, dividing this class of enzymes into Cu-/Zn-, Ni-, Mn-, and Fe-SODs. In this study, a superoxide dismutase from the thermophilic bacteria Thermobifida fusca (TfSOD) was cloned and expressed before the recombinant enzyme was characterized. The enzyme was found to be active for superoxide dismutation measured by inhibition of cytochrome c oxidation and the inhibition of the autoxidation of pyrogallol. Its pH-optimum was determined to be 7.5, while it has a broad temperature optimum ranging from 20 to 90 °C. Combined with the Tm that was found to be 78.5 ± 0.5 °C at pH 8.0, TfSOD can be defined as a thermostable enzyme. Moreover, the crystal structure of TfSOD was determined and refined to 1.25 Å resolution. With electron paramagnetic resonance spectroscopy, it was confirmed that iron is the metal co-factor of TfSOD. The cell potential (Em) for the TfSOD-Fe3+/TfSOD-Fe2+ redox couple was determined to be 287 mV.

SUBMITTER: Hamre AG 

PROVIDER: S-EPMC10520107 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature

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Initial characterization of an iron superoxide dismutase from Thermobifida fusca.

Hamre Anne Grethe AG   Al-Sadawi Rim R   Johannesen Kirsti Merete KM   Bisarro Bastien B   Kjendseth Åsmund Røhr ÅR   Leiros Hanna-Kirsti S HS   Sørlie Morten M  

Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry 20230919 7


Superoxide dismutases (SODs) are enzymes that catalyze the dismutation of the superoxide radical anion into O<sub>2</sub> and H<sub>2</sub>O<sub>2</sub> in a two-step reaction. They are ubiquitous to all forms of life and four different types of metal centers are detected, dividing this class of enzymes into Cu-/Zn-, Ni-, Mn-, and Fe-SODs. In this study, a superoxide dismutase from the thermophilic bacteria Thermobifida fusca (TfSOD) was cloned and expressed before the recombinant enzyme was cha  ...[more]

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