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Effect of serial passage in tissue culture on sequence of omp1 from Chlamydia trachomatis clinical isolates.

ABSTRACT: Previous epidemiological studies of Chlamydia trachomatis frequently have required expansion of isolates in tissue culture. The possibility that C. trachomatis omp1 might undergo mutation during such expansion has not been examined systematically. We found no differences in the omp1 sequences from 10 clinical specimens before and after 20 in vitro passages.

SUBMITTER: Stothard DR

PROVIDER: S-EPMC105264 | biostudies-literature | 1998 Dec

REPOSITORIES: biostudies-literature

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Effect of serial passage in tissue culture on sequence of omp1 from Chlamydia trachomatis clinical isolates.

Stothard D R DR Van Der Pol B B Smith N J NJ Jones R B RB

Journal of clinical microbiology 19981201 12

Previous epidemiological studies of Chlamydia trachomatis frequently have required expansion of isolates in tissue culture. The possibility that C. trachomatis omp1 might undergo mutation during such expansion has not been examined systematically. We found no differences in the omp1 sequences from 10 clinical specimens before and after 20 in vitro passages. ...[more]

PMID: 9817897

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