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An artificially split class 3 intein.

ABSTRACT: Inteins excise themselves from precursor polypeptides through protein splicing, joining N- and C-exteins with a peptide bond. Split inteins are expressed as separate polypeptides that undergo protein trans splicing (PTS). Here, we demonstrate PTS can be achieved using an artificially split class 3 intein. Because class 3 inteins use an internal initiating nucleophile near the C-extein junction, rather than the first residue of the intein, both catalytic nucleophiles are present on a single polypeptide. This results in a compact arrangement of catalytic nucleophiles for PTS compared to the standard arrangement for split class 1 inteins.

SUBMITTER: Ariagno TM

PROVIDER: S-EPMC10559147 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature

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An artificially split class 3 intein.

Ariagno Tia M TM Smetana John S JS Lennon Christopher W CW

microPublication biology 20230922

Inteins excise themselves from precursor polypeptides through protein splicing, joining N- and C-exteins with a peptide bond. Split inteins are expressed as separate polypeptides that undergo protein <i>trans</i> splicing (PTS). Here, we demonstrate PTS can be achieved using an artificially split class 3 intein. Because class 3 inteins use an internal initiating nucleophile near the C-extein junction, rather than the first residue of the intein, both catalytic nucleophiles are present on a singl ...[more]

PMID: 37811346

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