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Light sheet fluorescence microscopy of cleared human eyes.

ABSTRACT: We provide here a procedure enabling light sheet fluorescence microscopy (LSFM) of entire human eyes after iDISCO + -based clearing (ClearEye) and immunolabeling. Demonstrated here in four eyes, post-processing of LSFM stacks enables three-dimensional (3D) navigation and customized display, including en face viewing of the fundus similarly to clinical imaging, with resolution of retinal capillaries. This method overcomes several limitations of traditional histology of the eyes. Tracing of spatially complex structures such as anterior ciliary vessels and Schlemm's canal was achieved. We conclude that LSFM of immunolabeled human eyes after iDISCO + -based clearing is a powerful tool for 3D histology of large human ocular samples, including entire eyes, which will be useful in both anatomopathology and in research.

SUBMITTER: Darche M

PROVIDER: S-EPMC10564773 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature

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Light sheet fluorescence microscopy of cleared human eyes.

Darche Marie M Borella Ysé Y Verschueren Anna A Gantar Ivana I Pagès Stéphane S Batti Laura L Paques Michel M

Communications biology 20231010 1

We provide here a procedure enabling light sheet fluorescence microscopy (LSFM) of entire human eyes after iDISCO + -based clearing (ClearEye) and immunolabeling. Demonstrated here in four eyes, post-processing of LSFM stacks enables three-dimensional (3D) navigation and customized display, including en face viewing of the fundus similarly to clinical imaging, with resolution of retinal capillaries. This method overcomes several limitations of traditional histology of the eyes. Tracing of spatia ...[more]

PMID: 37816868

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