Ontology highlight
ABSTRACT:
SUBMITTER: Darche M
PROVIDER: S-EPMC10564773 | biostudies-literature | 2023 Oct
REPOSITORIES: biostudies-literature
Darche Marie M Borella Ysé Y Verschueren Anna A Gantar Ivana I Pagès Stéphane S Batti Laura L Paques Michel M
Communications biology 20231010 1
We provide here a procedure enabling light sheet fluorescence microscopy (LSFM) of entire human eyes after iDISCO + -based clearing (ClearEye) and immunolabeling. Demonstrated here in four eyes, post-processing of LSFM stacks enables three-dimensional (3D) navigation and customized display, including en face viewing of the fundus similarly to clinical imaging, with resolution of retinal capillaries. This method overcomes several limitations of traditional histology of the eyes. Tracing of spatia ...[more]