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Monitoring microbial communities using light sheet fluorescence microscopy.

ABSTRACT: Microbes often live in dense, dynamic, multi-species communities whose architecture and function are intimately intertwined. Imaging these complex, three-dimensional ensembles presents considerable technical challenges, however. In this review, I describe light sheet fluorescence microscopy, a technique that enables rapid acquisition of three-dimensional images over large fields of view and over long durations, and I highlight recent applications of this method to microbial systems that include artificial closed ecosystems, bacterial biofilms, and gut microbiota. I comment also on the history of light sheet imaging and the many variants of the method. Light sheet techniques have tremendous potential for illuminating the workings of microbial communities, a potential that is just beginning to be realized.

SUBMITTER: Parthasarathy R

PROVIDER: S-EPMC5963963 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature

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Monitoring microbial communities using light sheet fluorescence microscopy.

Parthasarathy Raghuveer R

Current opinion in microbiology 20171122

Microbes often live in dense, dynamic, multi-species communities whose architecture and function are intimately intertwined. Imaging these complex, three-dimensional ensembles presents considerable technical challenges, however. In this review, I describe light sheet fluorescence microscopy, a technique that enables rapid acquisition of three-dimensional images over large fields of view and over long durations, and I highlight recent applications of this method to microbial systems that include ...[more]

PMID: 29175679

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