Ontology highlight
ABSTRACT:
SUBMITTER: Trevino MA
PROVIDER: S-EPMC10570013 | biostudies-literature | 2023 Oct
REPOSITORIES: biostudies-literature
Treviño Miguel Á MÁ Pantoja-Uceda David D Laurents Douglas V DV Mompeán Miguel M
Nucleic acids research 20231001 18
The SARS-CoV-2 Nsp8 protein is a critical component of the RNA replicase, as its N-terminal domain (NTD) anchors Nsp12, the RNA, and Nsp13. Whereas its C-terminal domain (CTD) structure is well resolved, there is an open debate regarding the conformation adopted by the NTD as it is predicted as disordered but found in a variety of complex-dependent conformations or missing from many other structures. Using NMR spectroscopy, we show that the SARS CoV-2 Nsp8 NTD features both well folded secondary ...[more]